Regulatory

Part:BBa_K1723022:Design

Designed by: Cyril Pulver   Group: iGEM15_EPF_Lausanne   (2015-09-17)

CYC_0 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 197
    Illegal PstI site found at 19
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 13
    Illegal PstI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 13
    Illegal BamHI site found at 51
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 197
    Illegal PstI site found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 197
    Illegal PstI site found at 19
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter was specifically designed to be controlled by complexes constituted of dCas9_VP64 and gRNAs c3_0, c6_0 and c7_0. Since it has been demonstrated that inhibition prevails over activation when combining the co-expression of dCas9_VP64 and those gRNAs [1], this promoter can be used as a basic cellular computation tool.


Source

Synthesized as a G-Block

References

[1] Farzadfard, F., Perli, S.D., Lu, T.K., 2013. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas, ACS Synthetic Biology (ACS publications), http://pubs.acs.org/doi/pdf/10.1021/sb400081r (16.09.2015)