Regulatory
Part:BBa_K1723022:Design
Designed by: Cyril Pulver Group: iGEM15_EPF_Lausanne (2015-09-17)
CYC_0 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 13
Illegal XbaI site found at 197
Illegal PstI site found at 19 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 13
Illegal PstI site found at 19 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 13
Illegal BamHI site found at 51 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 13
Illegal XbaI site found at 197
Illegal PstI site found at 19 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 13
Illegal XbaI site found at 197
Illegal PstI site found at 19 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter was specifically designed to be controlled by complexes constituted of dCas9_VP64 and gRNAs c3_0, c6_0 and c7_0. Since it has been demonstrated that inhibition prevails over activation when combining the co-expression of dCas9_VP64 and those gRNAs [1], this promoter can be used as a basic cellular computation tool.
Source
Synthesized as a G-Block
References
[1] Farzadfard, F., Perli, S.D., Lu, T.K., 2013. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas, ACS Synthetic Biology (ACS publications), http://pubs.acs.org/doi/pdf/10.1021/sb400081r (16.09.2015)